The Serology Division provides routine and confirmatory testing for many common sexually transmitted diseases (STDs). Methods include nucleic acid amplification tests, enzyme immunoassays, Western Blot (protein immunoblot), flocculation, and particle agglutination. Measles serology testing is available on an out-sourced referral basis.
Chlamydia trachomatis/Neisseria gonorrhoeae/Trichomonas vaginalis (CT/GC/TV) Section
The CT/GC/TV section performs testing for Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis using a single specimen by mean of nucleic acid amplification technology (NAAT). Specimens for CT/GC may be of oral, cervical, vaginal, rectal, or urethral origin. TV testing is not available for specimens of oral or rectal origin. Specimens must be either swab or urine collected using a Gen-Probe Aptima Combo collection device.
HIV Serology Section
The HIV section screens for HIV using recombinant and synthetic antigen-based enzyme immunoassay (EIA). Specimens may be submitted as whole blood, serum or plasma. The preferred specimen is 3.5 ml of whole blood collected in a 13x100mm serum separator tube. Reactive specimens are retested and if repeatedly reactive are reflexed to a confirmatory test, the Western Blot. The OraSure® Western Blot is available to confirm oral fluid specimens collected using the OraSure® Oral Fluid Collection Device.
Syphilis Serology Section
The Syphilis section changed how it performs routine screening for syphilis. The screening procedure now utilizes an enzyme immunoassay (EIA) using recombinant antigens specific to Treponema pallidum. The preferred specimen is 3.5 ml of whole blood collected in a 13x100mm serum separator tube. Reactive specimens are reflexed to the VDRL (Venereal Disease Research Laboratory), which provides an indication as to the stage of infection and efficacy of treatment. If necessary, the specimen will be tested using a second treponemal test, the TP-PA (Treponema Pallidum-Particle Agglutination). The section also test spinal fluid by the VDRL method, please submit 1.0 - 1.5 ml of spinal fluid for the assay.
Measles Serology: Rubella, Rubeola
PCR testing is available via referral to the Wisconsin state Laboratory of Hygiene. The acceptable specimen is either a nasopharyngeal or throat swab in 2 ml of viral transport media. Submit and ship on ice pack (4°C). Requests for PCR testing should be made through the local county health department.
AIDS and HIV
Page last updated: April 12, 2017